CONTINUOUS PRODUCTION OF PURE SQUALENE BY USING COLUMN CHROMATOGRAPHY

Separation of aflatoxins by column chromatography.

Several column chromatographic methods are available for the separation of aflatoxins (l-6). Most of these procedures are elaborate and involve several steps of purification. A rapid and simple method of obtaining pure aflatoxins was reported by Steyn (5); however, with this method, we were not able to obtain pure crystalline aflatoxins B, and G1 for our experimental studies on animals. When pu...

Separation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column

The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...

Separation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column

The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...

Optimization of Continuous Flow Adsorption of Heavy Metal Ions on Continuous System Column by Peganum Harmala Seeds

Background & Aims of the Study: Heavy metals discharge to environment is a worldwide problem growing in scale. When they accumulate in the environment and in food chains, they can profoundly disrupt biological processes. Peganum Harmala Seeds (PHS) was used as a bio-sorbent, using a continuous system for removing Pb2+, Co2+, Ni2+ and Cu2+ ions from aqueous solutions. ...

Column Liquid Chromatography